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    首页 > 产品中心 > 一抗 > 产品信息
    Rabbit Anti-CD4 antibody (bs-0647R)
    ~~~Bioss促销~~~
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    说明书: 50ul  100ul  200ul
    20ul/480.00元
    50ul/880.00元
    100ul/1580.00元
    200ul/2480.00元
    大包装/询价

    产品编号 bs-0647R
    英文名称 CD4
    中文名称 CD4抗体
    别    名 CD4 (L3T4); CD4 antigen (p55); CD4 Antigen ; CD4 molecule; CD4 Receptor; CD4+ Lymphocyte deficiency, included; CD4mut; L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; MGC165891; p55; T Cell Antigen T4 ; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T cell OKT4 deficiency, included; T cell surface antigen T4/Leu 3 ; T cell surface antigen T4/Leu3; T Cell Surface Glycoprotein CD4; W3/25; W3/25 antigen; T-cell surface glycoprotein CD4 isoform 1 precursor; CD4_HUMAN.  
    Specific References  (2)     |     bs-0647R has been referenced in 2 publications.
    [IF=1.03] Smit, Francis Edwin, et al. "Does prolonged post-mortem cold ischemic harvesting time influence cryopreserved pulmonary homograft tissue integrity?." Cell and Tissue Banking: 1-14.  IHC-F ;  Human.  
    [IF=1.26] Jaime-Ramirez, Alena C., et al. "Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma." Molecular Therapy-Oncolytics 5 (2017): 87-96.  IHC-P ;  Mouse.  
    研究领域 肿瘤  细胞生物  免疫学  干细胞  转录调节因子  细胞表面分子  t-淋巴细胞  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Sheep, Guinea Pig, MACFA
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 48kDa
    细胞定位 细胞膜 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human CD4:385-457/457 <Cytoplasmic>
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010].

    Function:
    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

    Subunit:
    Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

    Subcellular Location:
    Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.

    Post-translational modifications:
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

    Similarity:
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    SWISS:
    P01730

    Gene ID:
    920

    Database links:

    南粤风采36选7最新开奖 www.gtxm.net

    Entrez Gene: 920 Human

    Entrez Gene: 12504 Mouse

    Omim: 186940 Human

    SwissProt: P06332 Mouse

    SwissProt: P01730 Human

    Unigene: 631659 Human

    Unigene: 2209 Mouse




    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

    此抗体可识别55KDⅠ型单链穿膜糖蛋白。 CD4分子是存在于大多数辅助/诱导T细胞表面的59kDa的糖蛋白。正常淋巴组织中CD4的表达数量多于CD8,此抗体主要用于标记辅助/诱导T细胞,与CD8单抗联合使用对外周血淋巴细胞分型。 CD4抗原是HLA-II类分子和人类免疫缺陷病毒(HIV)-爱滋病的受体,在35-50%外周血淋巴细胞-辅助和诱导T细胞(Th/Ti)和70-80%人胸腺细胞上表达,在人的单核细胞表面也有低密度的表达。 CD4抗原有膜结合型和可溶性两种形式。Th/Ti可辅助Ig产生和T细胞毒T细胞的作用。
    产品图片
    Sample:
    HL60 Cell Lysate at 40 ug
    Primary: Anti- CD4 (bs-0647R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 48 kD
    Observed band size: 55kD
    U937 Cell Lysate at 40 ug
    Primary: Anti- CD4 (bs-0647R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 48 kD
    Observed band size: 55kD
    Sample:
    Spleen (Mouse) Lysate at 40 ug
    Primary: Anti- CD4 (bs-0647R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 48 kD
    Observed band size: 55 kD
    Sample:
    Jurkat(Human) Cell Lysate at 30 ug
    Primary: Anti- CD4 (bs-0647R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 48 kD
    Observed band size: 55 kD
    Sample:
    HUVEC(Human) Cell Lysate at 30 ug
    Primary: Anti- CD4 (bs-0647R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 48 kD
    Observed band size: 55 kD
    Sample:
    Jurkat(Human) Cell Lysate at 30 ug
    Primary: Anti- CD4 (bs-0647R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 48 kD
    Observed band size: 53 kD
    Sample:
    Spleen (Mouse) Lysate at 40 ug
    Primary: Anti- CD4 (bs-0647R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 48 kD
    Observed band size: 55kD
    Sample:
    Lymph node(Mouse) Lysate at 40 ug
    Primary: Anti- CD4 (bs-0647R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 48 kD
    Observed band size: 55kD
    Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (bs-0647R) at 1:4000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (bs-0647R) at 1:4000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (bs-0647R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (bs-0647R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgical Research. Pig lymph nodes stained with Rabbit Anti-CD4 Polyclonal Antibody(bs-bs-0647R)at 1:300 for one hour at room temperature.
    Blank control (blue line): HL60 (fixed with 2% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
    Primary Antibody (green line): Rabbit Anti-CD4 antibody (bs-0647R),Dilution: 1μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,dilution: 1μg /test.
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